BS-010170-A03 | Eight position bioreactor for the non-invasive monitoring of cell growth in real-time by measurement of OD, pH and pO2 (RTS-8 Plus)

Key Features

• Patented Reverse-Spin® technology non-invasively optimises mixing and oxygenation for aerobic cell cultivation
• Measures OD, pH and pO2
• Eight individually-controlled bioreactors for rapid bioprocess optimisation
• Laser-based NIR optical system accurately determines OD levels to monitor cell growth kinetics
• Utilises 50 mL Falcon tubes with membrane filters to provide a working media volume of 3 mL - 50 mL
• Temperature control range from 15°C below ambient to +60°C
• Active cooling for rapid temperature control
• Real-time cell growth logging
• Software included for storage, demonstration and analysis of data in real-time
• Up to 12 RTS-8 Plus units can be simultaneously controlled by a PC through the software

Description

The RTS-8 Plus is an eight position bioreactor that utilises patented Reverse-Spin® technology to non-invasively optimise mixing and oxygenation for aerobic cell cultivation. With this novel technology a cell suspension in a single use 50 mL Falcon tube with a membrane filter is rotated around its axis in alternating directions to achieve a mass transfer coefficient (k_La (h^-1)) of up to 450. This level of mass transfer is ideal for efficient aerobic cell culture.

The RTS-8 Plus features a laser-based optical system that emits NIR light at 850 nm. Highly accurate measurements of OD can be taken from 1 - 60 times per hour to provide a profile of cell growth in real-time. The temperature control range for the RTS-8 Plus is 15°C below ambient to +60°C.

Key applications for the RTS-8 Plus include:

• Fermentation real time growth kinetics
• Clone candidate screening
• Protein expression
• Temperature stress and fluctuation experiments
• Media screening and optimisation
• Growth characterisation
• Inhibition and toxicity tests
• Strain quality control
• Initial bioprocess optimisation studies

Specifications

Light source	Laser
Measurement wavelength (λ)	850 ±15 nm
Measurement range	0 - 100 OD600
E. coli factory calibration measurement range	0 - 50 OD600
S. cerevisiae factory calibration measurement range	0 - 75 OD600
Achievable user calibration measurement error (range 0.1 - 6 OD600)	± 0.3
Achievable user calibration measurement error (range 6 - 50 OD600)	≤ 5%
Achievable user calibration measurement error (range 50 - 75 OD600)	≤ 10%
Measurement periodicity per hour	1 - 60
Temperature setting range	+15°C to +60°C
Temperature control range	15°C below ambient to +60°C
Temperature stability	±0.3°C
Sample temperature accuracy (20°C - 37°C)	±1°C
Tube sockets	8
Sample working volume range	3 mL - 50 mL
Speed control range	150 rpm - 2700 rpm
Reverse spin time setting range 150 rpm - 250 rpm	0 s
Reverse spin time setting range 250 rpm - 300 rpm	2 s - 60 s
Reverse spin time setting range 300 rpm - 2700 rpm	0 s - 60 s
Overall dimensions (W x D x H)	350 mm x 690 mm x 300 mm
Weight	20 Kg
Power supply	230 VAC: 50 Hz
Power consumption	3.15 A / 500 W

Sensor Specifications

O2 Sensor Specifications
Range	0 - 100%
Accuracy	±0.05% O2 at 0.2% ±0.4% O2 at 20.9%
Drift	<0.03% O2 within 30 days
Temperature range	Up to 40°C
Response time (t90)	<6 s
Storage stability	18 months
pH Sensor Specifications
Range	4.0 - 8.5 pH
Accuracy	±0.10 pH at pH 7
Drift	<0.005 pH per day
Operating temperature range	up to 40°C
Response time (t90)	<120 s
Storage stability	18 months

Back to home page