EXT1000 | ExTransfection™ electroporation transfection system with advanced microporation technology

Key Features

• High-efficiency transfection and superior viability
• Optimised for delivering nucleic acids, proteins and siRNA into mammalian cella, primary stem cells, and blood cells
• Ideal for CRISPR/Cas9 applications
• Utilises a novel capillary-type electric chamber to replace the conventional cuvettes seen with other systems
• Consistent electric field
• Gold-coated electrodes
• Optimised protocols | 300 cell-specific protocols available for download from the Protocol Library 
• Sample sizes: 10 μL and 100 μL
• Wide cell sample range from 10,000 to 10 million cells per reaction
• Wide range of kits (please see below)
  

Product Description

The NanoEntek ExTransfection™ Electroporation System is an innovative, benchtop electroporation device designed for the efficient transfection of cell lines, primary cells, stem cells, and hematopoietic cells. NanoEntek is the original developer of this microporation technology that is designed to overcome the limitations of traditional electroporation methods using cuvettes. 

The core of microporation technology lies in its use of a capillary-type electric chamber with a minimal electrode surface area in direct contact with the sample. It consists of a single electroporation chamber that delivers precise electrical pulses to ensure high transfection efficiency and cell viability by generating a strong, uniform electric field.

Ordering Information

Devices and Accessories

Part Number	Product	Contents
EXT1000	ExTransfection™ Electroporation System	Main device | Pipette | Pipette Station
EXT1000P	ExTransfection™ Pipette	ExTransfection™ Pipette (1 each)
EXT1000PS	ExTransfection™ Pipette Station	ExTransfection™ Pipette Station (1 each)

Consumables

Part Number	Product	Contents
EXT1025K	ExTransfection™ 10 µL kit (25 x 2 reactions)	Resuspension buffer R ( 1 mL) Resuspension buffer T (1 mL) Electrolytic buffer E (75 mL) Disposable tip (10 uL | 25 tips ExTransfection™ tube (5 each)
EXT10025K	ExTransfection™ 100 µL kit (25 x 2 reactions)	Resuspension buffer R ( 10 mL) Resuspension buffer T (10 mL) Electrolytic buffer E2 (75 mL) Disposable tip (100 uL | 25 tips ExTransfection™ tube (5 each)
EXT1096K	ExTransfection™ 10 µL kit (96 x 2 reactions)	Resuspension buffer R ( 1 mL: 3 each) Resuspension buffer T (1 mL: 3 each) Electrolytic buffer E (150 mL: 2 each) Disposable tip (10 uL | 96 tips ExTransfection™ tube (20 each)
EXT10096 K	ExTransfection™ 100 µL kit (96 x 2 reactions)	Resuspension buffer R ( 30 mL) Resuspension buffer T (30 mL) Electrolytic buffer E2 (150 mL : 2 each) Disposable tip (100 uL | 96 tips ExTransfection™ tube (20 each)
EXT50T	ExTransfection™ Tube	ExTransfection™ 10 µL kit (5 each: 10 packs)

More Information

If you're new to the field of Electroporation/Transfection then please click here to download an introductory brochure on this subject. Please click here to download a brochure describing the ExTransfection™ Electroporation System.

Specifications

Manufacturer	NanoEntek
Model number	EXT1000
Reaction Volume	10 µL - 100 µL
Cell concentrations	5 x 104 - 5 x 105 cells for 10 µL tip | 5 x 105 - 5 x 106 cells for 100 µL tip
Pulse voltage	500 V - 2500 V
Pulse width	1 ms - 100 ms
Number of pulses	1 - 10
User interface	20 cm touch screen
Electrical rating	100 VAC - 240 VAC | 4 A | 50/60 Hz
Electrical input	24 VDC | 7.5 A | 180 W
Instrument weight	6 Kg
Instrument dimensions	252 mm x 383 mm x 186 mm

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